2024年4月12日发(作者：)

Agilent DNF-493 HS Large

Fragment Kit Quick Guide for

Fragment Analyzer Systems

The Agilent Fragment Analyzer systems are automated capillary electrophoresis platforms for scalable, flexible, fast,

and reliable electrophoresis of nucleic acids.

This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The Large

Fragment kit (p/n DNF-493-0500 and DNF-493-1000) is designed for the sizing and quantification of small to mid-range

molecular weight dsDNA smears/fragments. Example applications include quality control of Next Generation Sequencing

(NGS) libraries.

Specifications

Analytical specifications

DNA Sizing Range

DNA Sizing Accuracy

1,2

DNA Fragment Concentration Range

1

DNA Smear Concentration Range

1

DNA Quantification Accuracy

1

DNA Quantification Precision

1

Maximum DNA Concentration

Physical Specifications

3

Total electrophoresis run time

Samples per run

Sample volume required

Kit stability

HS Large Fragment assay

75 bp – 20,000 bp

+ 15% or better

0.005 ng/µL – 0.5 ng/µL input DNA

0.05 ng/µL – 5 ng/µL input DNA

+ 25%

20% CV

0.5 ng/µL per fragment; 5 ng/µL per total sample

22cm

3

: 30 minutes, 33cm: 50 minutes, 55cm: 80 minutes

12, 48 or 96; depending on the instrument type

2 µL

4 months

1

Results using DNA Ladder of DNA fragment standards prepared in 1x TE buffer.

2

The 22 cm effective, 47 cm total length capillary is only available for 12-capillary Fragment Analyzer instruments

1

DNF-493 HS Large Fragment Quick Guide for Fragment Analyzer Systems

Kit Components – 500 Sample Kit

Kit Component

Number

5191-6589*

Part Number

(Re-order Number)

Description

HS Large Fragment, 500, 4

o

C

Quantity Per Kit

DNF-220-0240

DNF-300-0008

DNF-355-0125

Large Fragment Separation Gel, 240 mL

BF-25 Blank Solution, 8mL

5x 930 dsDNA Inlet Buffer, 125 mL

• Dilute with sub-micron filtered water prior

to use

0.25x TE Rinse Buffer, 125mL

1

1

1

DNF-497-0125 1

DNF-493-FR*

DNF-600-U030

DNF-381-0003

DNF-379-U100

HS Large Fragment, FR

Intercalating Dye, 30 μL

HS Large Fragment Diluent Marker, 2.4mL

HS Large Fragment DNA Ladder, 100µL

1

5

1

DNF-475-0050

5x Capillary Conditioning Solution, RT 1

• Dilute with sub-micron filtered water prior to use

2