2024年4月28日发(作者：)

Gene Designer by DNA 2.0 Tutorial

Prepared by: Angela Dixon

Table of Contents

Getting Started .............................................................................................................................................. 2

Library Explorer ............................................................................................................................................. 2

New Elements ........................................................................................................................................... 2

Design Toolbox .......................................................................................................................................... 4

Vectors ...................................................................................................................................................... 5

Codon Table Library .................................................................................................................................. 6

Codon Optimization ...................................................................................................................................... 8

Find Panel ...................................................................................................................................................... 9

Motifs ...................................................................................................................................................... 10

Restriction Sites ...................................................................................................................................... 10

ORF’s ....................................................................................................................................................... 10

Icon View ..................................................................................................................................................... 11

Sequence View ............................................................................................................................................ 11

Designing ..................................................................................................................................................... 14

Tools ............................................................................................................................................................ 14

Back Translate ......................................................................................................................................... 14

Cloning Tool ............................................................................................................................................ 15

Design sequencing oligos ........................................................................................................................ 17

Order gene/Request quote ..................................................................................................................... 17

Configure ..................................................................................................................................................... 18

Restriction Sites ...................................................................................................................................... 18

Back Translation Profiles ......................................................................................................................... 19

Motifs ...................................................................................................................................................... 19

Default Codon Table ............................................................................................................................... 20

1

Getting Started

-Go to

-Under the tab Scientific Resources, select Gene Designer

-Click on the Download Now icon

-Create an Account

-Click on the Install Now icon

-An activation key will be sent to your email address.

-Once you have it installed and activated, open Gene Designer

Library Explorer

The Library Explorer should automatically pop up when you open Gene Designer. It is a floating icon.

New Elements

Under the New Elements tab you have the options to add:

 New DNA Element

 New AA Element

 New ORF

 New Design Construct

 New Folder/Group

 New Codon Usage Table

2